Journal: BMC Genomics

Article Title: GlaI digestion of mouse γ-satellite DNA: study of primary structure and ACGT sites methylation

doi: 10.1186/1471-2164-10-322

Figure Lengend Snippet: Mouse genomic DNA cleavage with enzymes GlaI, Sse9I, FatI, Bst2UI . 1,8% low melting point agarose, Tris-acetate buffer. M – DNA marker pUC19/MspI (the lengths of the fragments are shown at right).

Article Snippet: The following DNA preparations were used as DNA fragment length markers: 1 kbp DNA ladder and pUC19/MspI DNA ladder (SibEnzyme Ltd., Russia).

Techniques: Marker

Journal: BMC Genomics

Article Title: GlaI digestion of mouse γ-satellite DNA: study of primary structure and ACGT sites methylation

doi: 10.1186/1471-2164-10-322

Figure Lengend Snippet: A physical map of mouse γ-satellite DNA . A – gel photo of mouse genomic DNA cleavage with restriction enzymes (double digestions). Lanes: 4 – intact DNA; 1, 3, 5, 7, 9 – GlaI added; 1, 2 – AcsI added; 5, 6 – FatI added; 7, 8 – BstF5I added; 9, 10 – Bst2UI added; M – DNA marker pUC19/MspI. B-F – physical maps of mouse γ-satellite DNA with indication of experimentally obtained DNA fragments and theoretically predicted ones. B – GlaI; C – double digestion with GlaI and AcsI; D – double digestion with GlaI and FatI; E – double digestion with GlaI and BstF5I; F – double digestion with GlaI and Bst2UI. Experimental patterns of mouse DNA hydrolysis and DNA ladders are shown on a left side of each figure. Positions of cleavage sites are shown in brackets at the top. Intact GlaI fragments are shown in black, cleaved GlaI fragments are shown in grey, double digestion products are shown in brown. Horizontal dotted lines show a correspondence of predicted fragments (arrows) to those observed on gel photos.

Article Snippet: The following DNA preparations were used as DNA fragment length markers: 1 kbp DNA ladder and pUC19/MspI DNA ladder (SibEnzyme Ltd., Russia).

Techniques: Marker

Journal: Molecules

Article Title: Synthesis and Evaluation of New Pyrazoline Derivatives as Potential Anticancer Agents

doi: 10.3390/molecules201019066

Figure Lengend Snippet: The DNA cleaving capability of the Fe(II) complexes of compound 11 and cisplatin in the presence of the iron (II) complex ( a ) and control experiments using compound 11 in the presence and absence of FeSO 4 , H 2 O 2 , and ascorbic acid ( b ). It was studied by the relaxation of the supercoiled pUC19 DNA and analyzed by agarose-gel electrophoresis.

Article Snippet: The DNA cleavage activities of the compounds were studied using supercoiled pUC19 DNA and analyzed by agarose (Takara, Kyoto, Japan) gel electrophoresis Mupid-2x (Mupid, Tokyo, Japan). pUC19 DNA (2 μg) was treated with compounds in water and Tris/boric acid (Nacalai Tesque, Kyoto, Japan) buffer (10 mM, pH 8.5) in the presence and absence of iron (II) sulfate heptahydrate (FeSO 4 ·7H 2 O; 30 μM) (Wako Pure Chemical Industries), hydrogen peroxide (H 2 O 2 ; 30 μM) (Tokyo Chemical Industry Co., Tokyo, Japan) and ascorbic acid (30 μM) (Tokyo Chemical Industry Co.) as an activator.

Techniques: Control, Agarose Gel Electrophoresis